| 质粒名称: | pBS NL4-3 envFS eGFP PR-D25A |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 101334 |
| 存储实验室: | Jeremy Luban |
|
载体骨架基本信息 |
|
| 载体名称: | pBluescript |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | NL4-3 envFS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | Protease D25A |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBS NL4-3 envFS eGFP PR-D25A was a gift from Jeremy Luban (Addgene plasmid # 101334 ; http://n2t.net/addgene:101334 ; RRID:Addgene_101334) |
| 参考文献部分: | Intron-containing RNA from the HIV-1 provirus activates type I interferon and inflammatory cytokines. McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl WE, Luban J. Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2. 10.1038/s41467-018-07753-2 PubMed 30546110 |
买家导航
pBS NL4-3 envFS eGFP PR-D25A载体质粒基本信息
pBS NL4-3 envFS eGFP PR-D25A质粒图谱载体图谱和pBS NL4-3 envFS eGFP PR-D25A载体序列质粒序列多克隆位点信息
pBS NL4-3 envFS eGFP PR-D25A质粒载体简介
pBS NL4-3 envFS eGFP PR-D25A质粒序列载体序列
LOCUS pBS NL4-3 envFS eGFP PR-D25A 12625 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic
activity.
ACCESSION .
VERSION .
KEYWORDS pBS NL4-3 envFS eGFP PR-D25A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12625)
AUTHORS McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl
WE, Luban J
TITLE Intron-containing RNA from the HIV-1 provirus activates type I
interferon and inflammatory cytokines.
JOURNAL Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.
PUBMED 30546110
REFERENCE 2 (bases 1 to 12625)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12625
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 377..634
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
CDS 790..2292
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/label=HIV-1 gag
/translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
CDS 2085..5096
/codon_start=1
/gene="pol"
/product="pol protein from human immunodeficiency virus 1"
/label=HIV-1 pol
/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLATGADDTVLEEMNLPGRWKPKMIG
GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
MAGDDCVASRQDED"
misc_feature 4781..4898
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
misc_feature 7761..7994
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8179..8223
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 8517..8543
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 8810..9526
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
CDS 8810..9523
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(8852..8873)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(9113..9132)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 9460..9481
/label=EGFP-C
/note="EGFP, forward primer"
LTR 9716..10349
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
rep_origin complement(10425..11013)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10505..10524)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11184..12044)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11807..11826
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12045..12149)
/gene="bla"
/label=AmpR promoter
rep_origin 12176..12604
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(12263..12282)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 12473..12494
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc
2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc
2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggag
2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgacccc
2281 tcgtcacaat aaagataggg gggcaattaa aggaagctct attagctaca ggagcagatg
2341 atacagtatt agaagaaatg aatttgccag gaagatggaa accaaaaatg atagggggaa
2401 ttggaggttt tatcaaagta agacagtatg atcagatact catagaaatc tgcggacata
2461 aagctatagg tacagtatta gtaggaccta cacctgtcaa cataattgga agaaatctgt
2521 tgactcagat tggctgcact ttaaattttc ccattagtcc tattgagact gtaccagtaa
2581 aattaaagcc aggaatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa
2641 taaaagcatt agtagaaatt tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg
2701 ggcctgaaaa tccatacaat actccagtat ttgccataaa gaaaaaagac agtactaaat
2761 ggagaaaatt agtagatttc agagaactta ataagagaac tcaagatttc tgggaagttc
2821 aattaggaat accacatcct gcagggttaa aacagaaaaa atcagtaaca gtactggatg
2881 tgggcgatgc atatttttca gttcccttag ataaagactt caggaagtat actgcattta
2941 ccatacctag tataaacaat gagacaccag ggattagata tcagtacaat gtgcttccac
3001 agggatggaa aggatcacca gcaatattcc agtgtagcat gacaaaaatc ttagagcctt
3061 ttagaaaaca aaatccagac atagtcatct atcaatacat ggatgatttg tatgtaggat
3121 ctgacttaga aatagggcag catagaacaa aaatagagga actgagacaa catctgttga
3181 ggtggggatt taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg
3241 gttatgaact ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaggaca
3301 gctggactgt caatgacata cagaaattag tgggaaaatt gaattgggca agtcagattt
3361 atgcagggat taaagtaagg caattatgta aacttcttag gggaaccaaa gcactaacag
3421 aagtagtacc actaacagaa gaagcagagc tagaactggc agaaaacagg gagattctaa
3481 aagaaccggt acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga
3541 agcaggggca aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa
3601 caggaaagta tgcaagaatg aagggtgccc acactaatga tgtgaaacaa ttaacagagg
3661 cagtacaaaa aatagccaca gaaagcatag taatatgggg aaagactcct aaatttaaat
3721 tacccataca aaaggaaaca tgggaagcat ggtggacaga gtattggcaa gccacctgga
3781 ttcctgagtg ggagtttgtc aatacccctc ccttagtgaa gttatggtac cagttagaga
3841 aagaacccat aataggagca gaaactttct atgtagatgg ggcagccaat agggaaacta
3901 aattaggaaa agcaggatat gtaactgaca gaggaagaca aaaagttgtc cccctaacgg
3961 acacaacaaa tcagaagact gagttacaag caattcatct agctttgcag gattcgggat
4021 tagaagtaaa catagtgaca gactcacaat atgcattggg aatcattcaa gcacaaccag
4081 ataagagtga atcagagtta gtcagtcaaa taatagagca gttaataaaa aaggaaaaag
4141 tctacctggc atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagataaat
4201 tggtcagtgc tggaatcagg aaagtactat ttttagatgg aatagataag gcccaagaag
4261 aacatgagaa atatcacagt aattggagag caatggctag tgattttaac ctaccacctg
4321 tagtagcaaa agaaatagta gccagctgtg ataaatgtca gctaaaaggg gaagccatgc
4381 atggacaagt agactgtagc ccaggaatat ggcagctaga ttgtacacat ttagaaggaa
4441 aagttatctt ggtagcagtt catgtagcca gtggatatat agaagcagaa gtaattccag
4501 cagagacagg gcaagaaaca gcatacttcc tcttaaaatt agcaggaaga tggccagtaa
4561 aaacagtaca tacagacaat ggcagcaatt tcaccagtac tacagttaag gccgcctgtt
4621 ggtgggcggg gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtaa
4681 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac
4741 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
4801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
4861 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
4921 gagatccagt ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa
4981 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc atcagggatt
5041 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattaacaca
5101 tggaaaagat tagtaaaaca ccatatgtat atttcaagga aagctaagga ctggttttat
5161 agacatcact atgaaagtac taatccaaaa ataagttcag aagtacacat cccactaggg
5221 gatgctaaat tagtaataac aacatattgg ggtctgcata caggagaaag agactggcat
5281 ttgggtcagg gagtctccat agaatggagg aaaaagagat atagcacaca agtagaccct
5341 gacctagcag accaactaat tcatctgcac tattttgatt gtttttcaga atctgctata
5401 agaaatacca tattaggacg tatagttagt cctaggtgtg aatatcaagc aggacataac
5461 aaggtaggat ctctacagta cttggcacta gcagcattaa taaaaccaaa acagataaag
5521 ccacctttgc ctagtgttag gaaactgaca gaggacagat ggaacaagcc ccagaagacc
5581 aagggccaca gagggagcca tacaatgaat ggacactaga gcttttagag gaacttaaga
5641 gtgaagctgt tagacatttt cctaggatat ggctccataa cttaggacaa catatctatg
5701 aaacttacgg ggatacttgg gcaggagtgg aagccataat aagaattctg caacaactgc
5761 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga
5821 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag
5881 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt
5941 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
6001 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta
6061 atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt
6121 gtgtggtcca tagtaatcat agaatatagg aaaatattaa gacaaagaaa aatagacagg
6181 ttaattgata gactaataga aagagcagaa gacagtggca atgagagtga aggagaagta
6241 tcagcacttg tggagatggg ggtggaaatg gggcaccatg ctccttggga tattgatgat
6301 ctgtagtgct acagaaaaat tgtgggtcac cgtctattat ggggtacctg tgtggaagga
6361 agcaaccacc actctatttt gtgcatcaga tgctaaagca tatatgatac agaggtacat
6421 aatgtttggg ccacacatgc ctgtgtaccc acagacccca acccacaaga agtagtattg
6481 gtaaatgtga cagaaaattt taacatgtgg aaaaatgaca tggtagaaca gatgcatgag
6541 gatataatca gtttatggga tcaaagccta aagccatgtg taaaattaac cccactctgt
6601 gttagtttaa agtgcactga tttgaagaat gatactaata ccaatagtag tagcgggaga
6661 atgataatgg agaaaggaga gataaaaaac tgctctttca atatcagcac aagcataaga
6721 gataaggtgc agaaagaata tgcattcttt tataaacttg atatagtacc aatagataat
6781 accagctata ggttgataag ttgtaacacc tcagtcatta cacaggcctg tccaaaggta
6841 tcctttgagc caattcccat acattattgt gccccggctg gttttgcgat tctaaaatgt
6901 aataataaga cgttcaatgg aacaggacca tgtacaaatg tcagcacagt acaatgtaca
6961 catggaatca ggccagtagt atcaactcaa ctgctgttaa atggcagtct agcagaagaa
7021 gatgtagtaa ttagatctgc caatttcaca gacaatgcta aaaccataat agtacagctg
7081 aacacatctg tagaaattaa ttgtacaaga cccaacaaca atacaagaaa aagtatccgt
7141 atccagaggg gaccagggag agcatttgtt acaataggaa aaataggaaa tatgagacaa
7201 gcacattgta acattagtag agcaaaatgg aatgccactt taaaacagat agctagcaaa
7261 ttaagagaac aatttggaaa taataaaaca ataatcttta agcaatcctc aggaggggac
7321 ccagaaattg taacgcacag ttttaattgt ggaggggaat ttttctactg taattcaaca
7381 caactgttta atagtacttg gtttaatagt acttggagta ctgaagggtc aaataacact
7441 gaaggaagtg acacaatcac actcccatgc agaataaaac aatttataaa catgtggcag
7501 gaagtaggaa aagcaatgta tgcccctccc atcagtggac aaattagatg ttcatcaaat
7561 attactgggc tgctattaac aagagatggt ggtaataaca acaatgggtc cgagatcttc
7621 agacctggag gaggcgatat gagggacaat tggagaagtg aattatataa atataaagta
7681 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
7741 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
7801 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctgatata
7861 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
7921 acagtctggg gcatcaaaca gctccaggca agaatcctgg ctgtggaaag atacctaaag
7981 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
8041 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaataa catgacctgg
8101 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
8161 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
8221 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
8281 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
8341 aggcagggat attcaccatt atcgtttcag acccacctcc caatcccgag gggacccgac
8401 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
8461 gtgaacggat ccttagcact tatctgggac gatctgcgga gcctgtgcct cttcagctac
8521 caccgcttga gagacttact cttgattgta acgaggattg tggaacttct gggacgcagg
8581 gggtgggaag ccctcaaata ttggtggaat ctcctacaat attggagtca ggagctaaag
8641 aatagtgctg ttagcttgct caatgccaca gccatagcag tagctgaggg gacagatagg
8701 gttatagaag tagtacaagg agcttgtaga gctattcgcc acatacctag aagaataaga
8761 cagggcttgg aaaggatttt gctataagat gggtggcgcg gccgctctgg tgagcaaggg
8821 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
8881 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
8941 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
9001 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
9061 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
9121 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
9181 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
9241 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
9301 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
9361 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
9421 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
9481 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaactcg agacctagaa
9541 aaacatggag caatcacaag tagcaataca gcagctaaca atgctgcttg tgcctggcta
9601 gaagcacaag aggaggaaga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
9661 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
9721 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
9781 ggctacttcc ctgattggca gaactacaca ccagggccag gggtcagata tccactgacc
9841 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
9901 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg gaatggatga ccctgagaga
9961 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
10021 catccggagt acttcaagaa ctgctgacat cgagcttgct acaagggact ttccgctggg
10081 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatgctgc
10141 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
10201 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
10261 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
10321 cttttagtca gtgtggaaaa tctctagcag gccggcccaa ttgacatgtg agcaaaaggc
10381 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
10441 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
10501 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
10561 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
10621 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
10681 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
10741 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
10801 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
10861 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
10921 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
10981 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
11041 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
11101 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
11161 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
11221 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
11281 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
11341 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
11401 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
11461 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
11521 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
11581 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
11641 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
11701 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
11761 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
11821 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
11881 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
11941 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
12001 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
12061 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
12121 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
12181 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
12241 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
12301 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
12361 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
12421 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
12481 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
12541 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
12601 aattttaaca aaatatttta attaa
//
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pBS NL4-3 envFS eGFP PR-D25A质粒载体应用举例
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